BOX-BEHNKEN DESIGN APPROACH TO DEVELOP NANO-VESICULAR HERBAL GEL FOR THE MANAGEMENT OF SKIN CANCER IN EXPERIMENTAL ANIMAL MODEL

Objective: To manage the increasing burden of skin cancer cases globally and to replace conventional invasive treatments their side effects, present study is aimed develop a transfersomal herbal gel Green Tea Catechins (GTC) extracted from indigenous green tea evaluate it for in vivo management an experimental animal model. Methods: GTC-loaded transfersomes (GTCTF) were prepared by thin-film hydration method. After optimizing GTCTFs using Box-Behnken design, they characterized zeta potential, structure, vitro drug release, permeation. Carbopol 940 was developed topical delivery GTCTF pH, viscosity, spreadability In MTT assay chemopreventive anticancer efficacy evaluated mice. Results: The has shown particle size 151.4±1.9 nm, entrapment efficiency 68.25±0.06 %, loading 10.41±0.02 %. B16F10 melanoma cell lines showed promising GTCTF. found suitable with favorable spreadability, permeability effective preventing curing mice, significant reduction tissue biochemical parameters like TNF-α, IL-1β, IL-6. Conclusion: Collectively, successful prevention induced model have established novel nanomedicine approach cancer.